FIG. 4.

Light micrographs of recombinant H. influenzae DB117 clones incubated with Chang conjunctival epithelial cells. Adherence assays were performed as described in Materials and Methods with the following modification: after the last wash to remove nonadherent bacterial cells, the monolayers were fixed for 10 min with methanol and subsequently stained with Giemsa stain. (A) DB117 carrying the cloning vector pACYC184; (B) DB117 carrying pELU112 (expressing 012E UspA1); (C) DB117 carrying pELU212 (expressing 012E UspA2); (D) DB117 carrying pELU246 (expressing 046E UspA2H).