TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference or Source |
|---|---|---|
| M. catarrhalis | ||
| 035E | Wild-type disease isolate, attaches to Chang cells, serum resistant | 2 |
| 035E.1 | uspA1 mutant of 035E, attachment deficient, serum resistant | 2 |
| 035E.2 | uspA2 mutant of 035E, attaches to Chang cells, serum sensitive | 2 |
| 035E.12 | uspA1 uspA2 mutant of 035E, attachment deficient, serum sensitive | 2 |
| 012E | Wild-type disease isolate, attaches to Chang cells, serum resistant | 1 |
| 012E.1 | uspA1 mutant of 012E, attachment deficient, serum resistant | This study |
| 012E.2 | uspA2 mutant of 012E, attaches to Chang cells, serum sensitive | This study |
| 012E.12 | uspA1 uspA2 mutant of 012E, attachment deficient, serum sensitive | This study |
| TTA37 | Wild-type disease isolate, attaches to Chang cells, serum sensitive | Steven Berk |
| TTA37.1 | uspA1 mutant of TTA37, attaches to Chang cells, serum sensitive | This study |
| TTA37.2 | uspA2H mutant of TTA37, attachment deficient, serum sensitive | This study |
| TTA37.12 | uspA1 uspA2H mutant of TTA37, attachment deficient, serum sensitive | This study |
| 046E | Wild-type disease isolate, attaches to Chang cells, serum resistant | John Nelson |
| 046E.1 | uspA1 mutant of 046E, attaches to Chang cells, serum resistant | This study |
| 046E.2 | uspA2H mutant of 046E, attaches to Chang cells, serum sensitive | This study |
| 046E.12 | uspA1 uspA2H mutant of 046E, attachment deficient, serum sensitive | This study |
| TTA24 | Wild-type disease isolate | 10 |
| P44 | Wild-type disease isolate | 25 |
| ATCC 25240 | Wild-type disease isolate | American Type Culture Collection |
| E22 | Wild-type disease isolate | 5 |
| V1166 | Wild-type isolate from nasopharynx of a healthy child | F. Henderson |
| V1171 | Wild-type isolate from nasopharynx of a healthy child | 10 |
| E. coli DH5α | Host strain for cloning experiments | 37 |
| H. influenzae DB117 | Host strain for cloning experiments | 39 |
| Plasmids | ||
| pBS KS(+) | Cloning vector, Ampr | Stratagene |
| pUSPA1KAN | pBS containing a truncated uspA1 gene from M. catarrhalis strain 035E into which a kanamycin resistance cartridge was inserted | 3 |
| pSPECr | Source of the spectinomycin resistance cartridge | 42 |
| pELU2P44SPEC | pBS containing an incomplete uspA2 gene from M. catarrhalis P44 into which a spectinomycin resistance cartridge was inserted | This study |
| pACYC184 | Cloning vector | New England Biolabs |
| pELU112 | pACYC184 containing the M. catarrhalis 012E uspA1 gene | This study |
| pELU212 | pACYC184 containing the M. catarrhalis 012E uspA2 gene | This study |
| pELU135 | pACYC184 containing the M. catarrhalis 035E uspA1 gene | This study |
| pELU235 | pACYC184 containing the M. catarrhalis 035E uspA2 gene | This study |
| pELU146 | pACYC184 containing the M. catarrhalis 046E uspA1 gene | This study |
| pELU246 | pACYC184 containing the M. catarrhalis 046E uspA2H gene | This study |
| pELU137 | pACYC184 containing the M. catarrhalis TTA37 uspA1 gene | This study |
| pELU237 | pACYC184 containing the M. catarrhalis TTA37 uspA2H gene | This study |
| pELU171 | pACYC184 containing the M. catarrhalis V1171 uspA1 gene | This study |
| pELU271 | pACYC184 containing the M. catarrhalis V1171 uspA2 gene | This study |
| pELU266 | pACYC184 containing the M. catarrhalis V1166 uspA2H gene | This study |