TABLE 4.
Adherence of recombinant H. influenzae cells to Chang conjunctival epithelial cells in vitro and detection of recombinant proteins on the bacterial cell surface
| Recombinant plasmid | M. catarrhalis proteina | Adherenceb | Detection of protein on cell surfacec |
|---|---|---|---|
| pACYC184 (vector) | 0.2 ± 0.1 | 918 ± 276 | |
| pELU112 | 012E-UspA1 | 35.1 ± 8.7 | 13,870 ± 2,132 |
| pELU212 | 012E-UspA2 | 0.4 ± 0.2 | 5,557 ± 1,546 |
| pELU135 | 035E-UspA1 | 17.4 ± 7.3 | 5,050 ± 1,184 |
| pELU235 | 035E-UspA2 | 0.2 ± 0.1 | 7,175 ± 3,201 |
| pELU171 | V1171-UspA1 | 12.3 ± 7.2 | 6,425 ± 1,049 |
| pELU271 | V1171-UspA2 | 0.4 ± 0.2 | 4,111 ± 1,333 |
| pELU137 | TTA37-UspA1 | 0.9 ± 0.4 | 1,286 ± 206 |
| pELU237 | TTA37-UspA2H | 16.9 ± 6.3 | 11,630 ± 1,351 |
| pELU146 | 046E-UspA1 | 0.8 ± 0.2 | 703 ± 221 |
| pELU246 | 046E-UspA2H | 52.6 ± 15.17 | 28,980 ± 1,703 |
| pELU266 | V1166-UspA2H | 62.3 ± 16.6 | 5,632 ± 199 |
a
The M. catarrhalis protein expressed by the H. influenzae DB117 clone containing this recombinant plasmid.
b
Adherence of M. catarrhalis organisms to Chang cells is expressed as the mean (± standard deviation) percentage of the inoculum which adhered to the monolayers.
c
Counts per minute (mean ± standard deviation) of radioiodinated goat anti-mouse immunoglobulin bound to MAb 17C7 on the surface of recombinant cells. The indirect antibody-accessibility assay was performed as described previously (2).