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. 2022 Sep 5;21:62. doi: 10.1186/s12938-022-01027-1

Table 3.

The primers and probe sequences

Primers Sequence (5ʹ–3ʹ) Annealing temperature
E_Sarbeco_F1 5ʹ-ACAGGTACGTTAATAGTTAATAGCGT-3ʹ 60 °C
E_Sarbeco_R2 5ʹ-ATATTGCAGCAGTACGCACACA-3ʹ
E_Sarbeco_P1 5ʹ-ACACTAGCCATCCTTACTGCGCTTCG-3ʹ