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Table 4.
The RT-qPCR setup
| Process | Temperature | Time | Cycles |
|---|---|---|---|
| RT | 50 °C | 15 min | 1 |
| Polymerase activation | 95 °C | 2 min | |
| PCR | 95 °C | 15 s | 40 |
| 60 °C | 30 s |