TABLE 1.

Genetic identification^a of biofilm-defective mutants of S. gordonii Challis

Mutant	Allele	Locus	% of level of wild-type biofilm formationb	Genetic function	Putative role in biofilm	Nucleotide sequence at Tn916 junctionc	Accession no.	Reference
1	8F9	comD	61.4	Histidine kinase	Quorum sensing	TTAATTTTATTGTAA	U80077	42
2	1C1	PBP 2B gene	9.0	Peptidoglycan biosynthesis	Osmoregulation?	AAGATTTTTATACTA	AF207585	This study
3	11E5L	PBP 5 gene	44.3	Peptidoglycan biosynthesis	Osmoregulation?	ATTATTTTTTAAAGA	AF207578	This study
	11E5U	ATPase gene		Cation transport	Osmoregulation?	CTATATTTTTAAAGA	AF207579	This study
4	11B4	glmM	55.5	Peptidoglycan biosynthesis	Osmoregulation?	GTTATTTTTTTATTA	AF207577	This study
5	15B3	bacA	12.6	Peptidoglycan biosynthesis	Osmoregulation?	TACTTTTATACTTTA	AF207587	This study
6	9F8	abpA	16.2	α-Amylase binding	Adhesion	CTCCTTTTTTATGTA	AF035817	51
7	29E5	appC	24.9	Oligopeptide transport	Nutrient sensing?	AGTTTTTTTTATGTA	AF207584	This study
8	4B3	mutT	17.1	DNA replication	Unknown	TTTATTATTAATTTA	AF207581	This study
9	13A12	ytmP	14.6	Unknown	Unknown	TTATTTTTTTTATTA	AF207592	This study
10	8A11	No match	35.3	Unknown	Unknown	GTATTTTTTACAAAA	AF207586	This study
11	6C12	No match	28.2	Unknown	Unknown	ATAATTTTTTATTTA	AF207591	This study
12	14H6	No match	26.1	Unknown	Unknown	GTTCTTTTTATTCAA	AF207582	This study
13	8B8	No match	33.2	Unknown	Unknown	TGATTTTTTTGAATA	AF207583	This study
14	12E11	No match	18.5	Unknown	Unknown	CTATATTTTTAAAGA	AF207576	This study
15	8G2	No match	21.6	Unknown	Unknown	CGAATTTTAATTTTA	AF207580	This study
16	29F1	No match	43.3	Unknown	Unknown	GTTCTTTTTAATGTA	AF207590	This study
17	18D10	No match	22.1	Unknown	Unknown	GCTATTATTATACCA	AF207588	This study
18	11G1	No match	32.1	Unknown	Unknown	TCGATTTTTTTATTA	AF207589	This study

^aBased on DNA homologies with DNA sequences in GenBank. 

^bWild-type biofilm formation was considered to be 100%. 

^cFifteen nucleotides upstream of the chromosomal DNA of the Tn916 insertion.