Table 2. Quenching Ratios and Unstacked Populations for C(2-AP)C^a.

		buffer			30% ethanol
smoothing	ε	Rϕ	% un	% fl un	Rϕ	% un	% fl un
none	1	22 ± 4	5.9 ± 0.7	58	6 ± 1	11 ± 4	42
5 nm	1	22 ± 1	6.0 ± 0.3	59	5.7 ± 0.5	11 ± 2	42
none	2	23 ± 4	6.2 ± 0.6	61	6 ± 1	12 ± 3	48
5 nm	2	23 ± 1	6.3 ± 0.2	61	6.2 ± 0.5	12 ± 1	47

^aResults are shown for models with and without smoothing by a 5 nm rolling average, and with (ε = 2) and without (ε = 1) accounting for hypochromicity of 2-AP in the stacked conformation. R_ϕ = quenching ratio (ϕ_un/ϕ_st); % un = percent unstacked; % fl un = % of total fluorescence that comes from the unstacked population. Mean ± standard deviation of the results obtained at all data points in the range of 300–320 nm.