TABLE 3.
MICs of nickel and cobalt in various derivatives of Ralstonia sp. strain AE126(pMOL28)
| Bacterial strain | Relevant genotype | MIC (mM)a
|
|
|---|---|---|---|
| Ni2+ | Co2+ | ||
| AE126(pMOL28) | Wild type | 4.0 | 5.0 |
| AE104 | Plasmid-free control | 0.3 | 0.3 |
| DN177(pMOL28-2) | Φ(cnrCBA-lacZ) | 4.0 | 5.0 |
| DN177(pMOL28-2)b | Φ(cnrCBA-lacZ) | 10 | |
| DN177(pMOL28-2, pDNA291) | Φ(cnrCBA-lacZ), cnrYXH in trans | 5.0 | 3.0 |
| DN190(pMOL28-3) | ΔcnrYXH Φ(cnrCBA-lacZ) | 0.4 | 0.3 |
| DN190(pMOL28-3, pDNA291) | ΔcnrYXH, cnrYXH in trans | 4.0 | 5.0 |
| DN195(pMOL28-4) | cnrY1(Con) | 7.0 | 10 |
| DN195(pMOL28-4, pDNA291) | cnrY1(Con), cnrYXH in trans | 7.0 | 10 |
a
The MIC is defined as the minimal concentration of heavy metal cation inhibiting growth at 30°C for 3 days. Occurrence of single colonies was not counted as growth. Each determination was repeated twice with identical results.
b
Cultivated in the presence of cobalt plus 1 mM Ni2+ for induction.