TABLE 2.
Probing promoters cnrYXHa
| Plasmid | Cloned fragment | Maximum fold induction with nickelbd | Transcription | Relative signal strength (%)cd |
|---|---|---|---|---|
| pMOL1551 (1–2418) | 1,027 (11) | Inducible | 100 (0.7) | |
| pMOL1550 (1–2298) |  |
1,036 (25) | Inducible | 100 |
| pMOL1561 (1–2298) | <2 | Constitutive | 13 (2.0) | |
| pMOL1586 (870–1720) | 210 (12) | Inducible | 23 (3.5) | |
| pMOL1593 (843–1256), (1256–1720) | <2 | Constitutive | 15 (3.8) | |
| pMOL1588 (1579–2298) | <2 | Constitutive | 58 (9.2) | |
| pMOL1591 (1253–1720) | <2 | Constitutive | 17 (2.8) | |
| pMOL1592 (870–1280) | <2 | Constitutive | 11 (0.7) | |
| pMOL1583 (870–1000) | <2 | Constitutive | 13 (2.8) | |
| pMOL1587 (1579–1720) | <2 | Constitutive | 17 (3.5) | |
| pMOL1596 (2280–2418) | <2 | Constitutive | 0.4 (0.07) | |
| pMOL877 | 0 | Inactive | 0.0 |
a
Transcriptional fusions between different regions of the cnr regulatory locus and the luxCDABE genes were tested for their metal-responsive activity in vivo in CH34.
b
The metal response to 0.3 mM nickel was quantitated as the fold induction compared to noninduced cells and is expressed as the signal-to-noise ratio. A value of <2 is considered constitutive transcription.
c
Maximum light production (in ALU) divided by the OD660nm of the culture, as compared to the transcription of the complete cnr regulatory locus from pMOL1550.
d
Standard deviations are indicated in parentheses and are based on the results from three independent experiments.