Fig. 2.

Nutlin-3 elevated the sensitivity of multiple breast cancer cell lines to THZ1. A, D CCK8 assay was utilized to measure the viability of ZR-75-1 or DU4475 cells with increasing concentrations of nutlin-3 for 24 h or 48 h. B, E CCK8 assay was utilized to measure the viability of ZR-75-1 or DU4475 cells with increasing concentrations of THZ1 for 24 h or 48 h. C, F The cell viability was detected by CCK8 assay after ZR-75-1 or DU4475 cells were treated with 10 nM or 20 nM nutlin-3 combining with 50 nM THZ1 for 24 h, 48 h or 72 h separately. G The colony formation assay was meant to test the ability of ZR-75-1 cell with 10 nM or 20 nM nutlin-3 combining with 50 nM THZ1 to form colony spots. H Quantitation of colony formation ability in G. I The protein levels of cleaved PARP and p53 in ZR-75-1 cell were detected by western blot. J Quantity of cleaved PARP and P53 severally in I. K The protein levels of cleaved PARP and p53 in DU4475 cell were detected by western blot. L Quantity of cleaved PARP and P53 severally in K. Statistically significant (P < 0.05) are *, (P < 0.01) are **