TABLE 1.
Bacterial and plasmid strains used in this study
| E. coli strain or plasmid | Relevant genotype | Source or reference |
|---|---|---|
| Strains | ||
| AB1157 | argE3 hisG4 leuB6 proA2 thr-1 ara-14 galK2 lacY1 mtl-1 xyl-1 thi-1 ara-1 rpsL31 supE44 tsx-33 | B. Bachman (4) |
| AK3 | recD1903::mini-tet | A. Kuzminov (11) |
| AM115 | CC105 recF322::Tn3 (Apr) | P1.JAS34 X CC105 to Apr; screen for UVS |
| AM116 | CC105mutA recF322::Tn3 (Apr) | P1.JAS34 X CC105mutA to Apr; screen for UVS |
| AM117 | CC105 recO1504::Tn5 (Kanr) | P1.JAS20 X CC105 to Kanr; screen for UVS |
| AM118 | CC105mutA recO1504::Tn5 (Kanr) | P1.JAS20 X CC105mutA to Kanr; screen for UVS |
| AM119 | CC105 recR252::Tn10-9 (Kanr) | P1.JAS31 X CC105 to Kanr; screen for UVS |
| AM120 | CC105mutA recR252::Tn10-9 (Kanr) | P1.JAS31 X CC105mutA to Kanr; screen for UVS |
| CC105 | [ara Δ(lac proB) xiii] F′ lacI Z proB+ | J. Miller (14) |
| CC105mutA | mutA590C in CC105 | J. Miller (14) |
| GS1481 | ΔruvC64::Kan (Kanr) in AB1157 | R. G. Lloyd (13) |
| JAS34a | recF322::Tn3(Apr) | J.A. Sawitzke (25) |
| JAS20a | recO1504::Tn5 (Kanr) | J. A. Sawitzke (25) |
| JAS31a | recR252::Tn10-9 (Kanr) | J. A. Sawitzke (25) |
| KH2R | Δ(srlR-recA)306::Tn10 (Tetr) in KH2 | This laboratory (20) |
| LR300 | recB268::Tn10 (Tetr) in CC105 | This laboratory (23) |
| LR400 | recB268::Tn10 (Tetr) in CC105mutA | This laboratory (23) |
| LR500 | recC266::Tn10 (Tetr) in CC105 | P1.N2103 X CC105 to Tetr; screen for UVS |
| LR600 | recC266::Tn10 (Tetr) in CC105mutA | P1.N2103 X CC105mutA to Tetr; screen for UVS |
| LR700 | recD1903::mini-tet (Tetr) in CC105 | P1.AK3 X CC105 to Tetr; screen for absence of Exo V activity |
| LR800 | recD1903::mini-tet (Tetr) in CC105mutA | P1.AK3 X CC105mutA to Tetr; screen for absence of Exo V activity |
| LR110 | ruvA60::Tn10 (Tetr) in CC105 | P1.N2507 X CC105 to Tetr; screen for UVS |
| LR120 | ruvA60::Tn10 (Tetr) in CC105mutA | P1.N2507 X CC105mutA to Tetr; screen for UVS |
| LR130 | ΔruvC64::Kan (Kanr) in CC105 | P1.GS1481 X CC105 to Kanr; screen for UVS |
| LR140 | ΔruvC64::Kan (Kanr) in CC105mutA | P1.GS1481 X CC105mutA to Kanr; screen for UVS |
| N2103 | recC266::Tn10 (Tetr) in AB1157 | R. G. Lloyd (12) |
| N2507 | ruvA60::Tn10 (Tetr) in AB1157 | R. G. Lloyd (26) |
| Plasmids | ||
| pBR322 | Apr Tetr (vector) | R. Brent (2) |
| pDWS2 | pBR322 derivative harboring recB, recC, and recD genes | A. Kuzminov (22) |
a
Other markers used were recB21 recC22 sbcB15 sbcC201sup0 hsdR ara-14 Δ(gpt-proA)62 lacY1 tsx-33 galK2 hisG4 rfbD1 mgl-51 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 Rac− F− λ−.