FIG. 2.

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of purified MalK wild-type and mutant proteins. A total of 5 μg of protein was loaded in each well, electrophoresed, and stained as described in reference 17. Lanes: 1, molecular mass standards; 2, wild type, prepared as described in reference 19; 3, L86F mutant; 4, wild type, prepared as described in reference 16; 5, P160L mutant; 6, D165N mutant; 7, wild type (His₆-MalK); 8, His₆-E306K mutant.