FIG. 5.
(A) ATPase activity of the complex containing the MalK L86F variant incorporated into liposomes. Proteoliposomes containing wild-type or mutant transport complexes were formed in the presence (filled symbols) or absence (open symbols) of maltose and maltose-binding protein (MalE) and assayed for ATPase activity as described in reference 7. Symbols: ○ and ●, wild-type complex; □ and ■, MalFG(KL86F)2. (B) Immunochemical detection of the individual subunits in the octylglucoside-solubilized fractions from membranes containing wild-type or mutant complexes. The reactions corresponding to MalK (K), MalF (F), and MalG (G) are indicated by arrows.