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Swarming abilities of HCB339 (ΔMCP) cells expressing wild-type (WT) and mutant Tcp receptors in tryptone semisolid agar which does not contain citrate. Aliquots (1 μl each) of fresh overnight cultures were inoculated onto tryptone semisolid agar (1% tryptone, 0.5% NaCl, 0.3% agar) supplemented with 25 μg of chloramphenicol per ml, and then the plate was incubated at 30°C for 14 h.
