R. capsulatus |
PAS100 |
Rfr Smr; str-2 hsd-1 restriction-deficient strain derived from wild-type B10 |
28 |
MC101 |
Rfr Kmr; derived from PAS100; Kmr cartridge inserted in cycP and transcribed in the opposite direction from cycP
|
This study |
MC111 |
As MC101, but Kmr gene transcribed in same direction as cycP
|
This study |
E. coli |
DH5α |
supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1
|
Gibco BRL |
TOP10F′ |
F′ (lacIq Tetr) mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7679 galU galK rpsL endA1 nupG
|
Invitrogen |
S17-1 |
thi pro hsdR recA RP4-2 integrated Tc::Mu Km::Tn7 (mobilizable vector) |
24 |
Plasmids |
pZErO |
Kmr
|
Invitrogen |
pGEM-3Zf(+) |
Apr
|
Promega |
pUC4-K |
Apr Kmr; source of Tn903-derived Kmr cartridge |
Pharmacia |
pRVS1 |
Spr Apr; mobilizable vector |
26 |
pCP101 |
Kmr; 6-kb SalI fragment containing cycP in pZErO |
This study |
pCP201 |
pCP101 with Kmr cartridge in XhoI site within cycP; Kmr inserted in opposite direction from transcription of cycP
|
This study |
pCP202 |
As pCP201 with Kmr cartridge inserted in same direction as transcription of cycP
|
This study |
pCP301 |
cycP::Kmr insert from pCP201 cloned into pRVS1 |
This study |
pCP302 |
cycP::Kmr insert from pCP202 cloned into pRVS1 |
This study |
pRK415 |
Broad-host-range, mobilizable vector; Tcr
|
16 |
pRKMC401 |
pRK415 containing BamHI fragment containing cycP
|
This study |