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. 2000 Mar;182(5):1442–1447. doi: 10.1128/jb.182.5.1442-1447.2000

TABLE 1.

Bacterial strains and plasmids used

Strain or plasmid Genotype and description Source or reference
R. capsulatus
 PAS100 Rfr Smr; str-2 hsd-1 restriction-deficient strain derived from wild-type B10 28
 MC101 Rfr Kmr; derived from PAS100; Kmr cartridge inserted in cycP and transcribed in the opposite direction from cycP This study
 MC111 As MC101, but Kmr gene transcribed in same direction as cycP This study
E. coli
 DH5α supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 Gibco BRL
 TOP10F′ F′ (lacIq Tetr) mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7679 galU galK rpsL endA1 nupG Invitrogen
 S17-1 thi pro hsdR recA RP4-2 integrated Tc::Mu Km::Tn7 (mobilizable vector) 24
Plasmids
 pZErO Kmr Invitrogen
 pGEM-3Zf(+) Apr Promega
 pUC4-K Apr Kmr; source of Tn903-derived Kmr cartridge Pharmacia
 pRVS1 Spr Apr; mobilizable vector 26
 pCP101 Kmr; 6-kb SalI fragment containing cycP in pZErO This study
 pCP201 pCP101 with Kmr cartridge in XhoI site within cycP; Kmr inserted in opposite direction from transcription of cycP This study
 pCP202 As pCP201 with Kmr cartridge inserted in same direction as transcription of cycP This study
 pCP301 cycP::Kmr insert from pCP201 cloned into pRVS1 This study
 pCP302 cycP::Kmr insert from pCP202 cloned into pRVS1 This study
 pRK415 Broad-host-range, mobilizable vector; Tcr 16
 pRKMC401 pRK415 containing BamHI fragment containing cycP This study