FIGURE 7.

hiPSC-CMs recorded in standard and fluoride-free internal solution. hiPSC-CMs were captured with almost 60% success rate for R_Seal ≥ 0.25 GΩ when using standard internal solution and about 25% R_Seal ≥ 0.25 GΩ in fluoride-free internal solution in preliminary experiments. The inset shows an example of a Ca_V current recorded from an example cell in standard (top, dark blue) and fluoride-free (bottom, light blue). The voltage protocol used is also shown and only response to voltage step to 0 mV is shown in the raw trace examples.