TABLE 3.
Parameters for RSeal, IPeak, rundown, Vhalf Activation, Vhalf Inactivation, and tetracaine IC50 for CHO cells expressing NaV1.5 for standard internal and fluoride-free internal solution.
| Parameter | Standard internal | Fluoride-free internal |
| RSeal (GΩ) | 1.89 ± 0.02 (3598) | 1.39 ± 0.02 (2061)*** |
| IPeak (nA) | −1.56 ± 0.01 (4537) | −1.53 ± 0.01 (3336) |
| Capacitance (pF) | 13.9 ± 0.2 (1043) | 13.8 ± 0.4 (491) |
| RSeries (MΩ) | 7.6 ± 0.1 (1043) | 9.8 ± 0.1 (491)*** |
| Rundown over 12 min (%/min; Vhold −120 mV) | 0.5 ± 0.1 (133) | 0.4 ± 0.1 (52) |
| Vhalf Activation (mV) | −37.5 ± 0.4 (484) | −25.2 ± 0.5 (241)*** |
| Vhalf Inactivation (mV) | −68.4 ± 0.4 (728) | −53.6 ± 0.6 (165)*** |
| IC50 Tetracaine (μM, Vhold −120 mV) | 35.6 ± 9.4 (4 chips) | 41.8 ± 9.3 (3 chips) |
RSeal values > 4 GΩ could not be accurately calculated, therefore only RSeal ≤ 4 GΩ were used for the calculation. Shown are mean ± S.E.M, number of cells shown in parentheses. For tetracaine concentration response curves, single point additions were made and the concentration response curve calculated across the whole chip. Shown are mean ± S.E.M for the number of chips given in parentheses.
***P < 0.001, unpaired Student’s t-test.