TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype | Constructiona or source |
|---|---|---|
| Bacillus strains | ||
| PY22 | trpC2 | P. Youngman |
| BSA46 | trpC2 SPβ ctc::lacZ | 3 |
| XS352 | trpC2 aph3′5"/sigB rsbST | 18 |
| BSA419 | trpC2 Pspac::rsbT SPβ ctc::lacZ | 17 |
| BSJ13 | trpC2 Pspac::rsbT Pxyl::obg SPβ ctc::lacZ | 17 |
| BSJ38 | trpC2 Pspac::rsbT rsbX::spec SPβ ctc::lacZ | BSA625 → BSA419 |
| BSJ39 | trpC2 aph3′5"/sigB ΔrsbST | XS352 → PY22 |
| BSJ40 | trpC2 aph3′5"/sigB ΔrsbST Pspac::rsbT SPβ ctc::lacZ | pHV501T → BSJ39 |
| BSJ41 | trpC2 aph3′5"/sigB ΔrsbST Pspac::rsbT SPβ ctc::lacZ | BSA46 → BSJ40 |
| BSJ42 | trpC2 aph3′5"/sigB rsbRΔ5 Pspac::rsbT SPβ ctc::lacZ | pJM49 → BSA419 |
| Plasmids | ||
| pHV501T | Apr Emr Pspac::rsbT | 17 |
| pRS11 | Apr KanrPA::rsbR rsbS | 18 |
| pJM49 | Apr KanrPA::rsbRΔ5 rsbS | This study |
| pML7/X::spec | Apr Cmr SprPBrsbVW sigB rsbX::spec | 4 |
a
Transformations were performed as described by Yasbin et al. (29).