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. Author manuscript; available in PMC: 2022 Sep 5.
Published in final edited form as: J Comp Neurol. 2017 Mar 10;527(1):225–235. doi: 10.1002/cne.24172

Figure 2. Satb1 and Satb2 are expressed in GFP-labeled On-Off DS RGCs but not in other defined RGC types.

Figure 2.

(A-J) Sections through P8 eyes from BAC transgenic GFP lines were immunostained with antibodies against Satb1 and Satb2. Anti-GFP antibodies were used to increase signal and sections were treated with DAPI (blue) to visualize cell nuclei. Nearly all DRD4-GFP RGCs express Satb1 (92%; n=57) and Satb2 (96%; n=52). TRHR-GFP RGCs also express both Satb1 (95%; n=114) and Satb2 (94%; n=50). HB9-GFP RGCs express Satb1 (92%; n=51) but not Satb2 (3%; n=78) (E-F). Very few CB2-GFP RGCs were labeled by Satb1 (1%; n=85) or Satb2 (12%; n=74) and similarly, very few Cdh3-GFP RGCs expressed Satb1 (1.5%; n=66) or Satb2 (1.1%; n=85)(G-J). (K) Graphical representation of A-J. ONL, Outer nuclear layer; IPL, inner plexiform layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar: 20 uM in A for panels A-J.