Table 1. Estimated thickness and calculated defocus per image.

Lamella #	Image file	Est thickness (nm)	Defocus 1 (Å)	Defocus 2 (Å)	Angle
1	24_Mar11_13.30.44_1_0.mrc	179	5577	5434	7.9
1	25_Mar11_13.32.53_3_0.mrc	170	5943	5636	53.07
2	50_Mar11_15.04.14_36_0.mrc	132	3626	3298	23.33
2	51_Mar11_15.07.03_38_0.mrc	109	3944	3863	16.91
2	52_Mar11_15.10.54_40_0.mrc	117	6491	6192	10.89
2	53_Mar11_15.21.33_42_0.mrc	205	4906	4610	5.32
2	54_Mar11_15.24.09_44_0.mrc	205	7701	6828	–76.32
2	55_Mar11_15.26.04_46_0.mrc	212	5141	4794	23.45
2	56_Mar11_15.33.15_48_0.mrc	170	7240	7093	82.03
2	57_Mar11_15.36.27_50_0.mrc	178	5969	5807	–7.87
2	58_Mar11_15.38.03_52_0.mrc	186	6445	6182	–75.84
3	115_Mar12_10.39.05_93_0.mrc	136	3436	3230	–22.72
3	118_Mar12_10.46.25_99_0.mrc	141	3291	3200	39.71
4	131_Mar12_11.32.55_127_0.mrc	114	4532	4377	15.85
4	133_Mar12_11.37.56_131_0.mrc	130	3252	2979	7.24
4	135_Mar12_11.42.53_135_0.mrc	93	3041	2977	–76.83
4	138_Mar12_11.50.12_141_0.mrc	106	3497	3404	79.97
5	141_Mar12_11.57.31_147_0.mrc	161	6153	5600	–33.24
5	143_Mar12_12.02.15_151_0.mrc	152	4552	4457	–36.2
5	146_Mar12_12.19.02_157_0.mrc	98	3902	3868	61.58
5	147_Mar12_12.21.27_159_0.mrc	103	4305	4066	16.81
5	148_Mar12_12.23.52_161_0.mrc	73	3728	3604	–36.33
5	149_Mar12_12.26.15_163_0.mrc	78	3696	3573	0.37
5	150_Mar12_12.28.45_165_0.mrc	98	3723	3607	1.6
5	151_Mar12_12.31.16_167_0.mrc	114	3771	3648	54.64
6	168_Mar12_13.11.12_199_0.mrc	147	3157	3040	–57.66
6	171_Mar12_13.18.43_205_0.mrc	90	2309	2102	31.47
7	6 A_Mar11_14.59.49_34_0.mrc	126	5210	4391	2.1