TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype and/or relevant characteristics | Reference or source |
|---|---|---|
| E. coli | ||
| DH5αF′ | recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 Δ(lacZYA-argF)U169 [Φ80dlacZΔM15] Nalr | 44 |
| M15 | lac1qara-14 galK2 mtl-1 F− | 49 |
| MC4100 | araD139 rpsL150 relA1 flbB5301 deoC1 pstF25 rbsR Δ(lacZYA-argF)U169 Strr | 5 |
| P. aeruginosa | ||
| PAO1 (ATCC 15692) | Prototroph | American Type Culture Collection |
| 8830 | his-1 | 9 |
| 8830R2::Cm | his-1 algR2::Cm | 42 |
| P. putida | ||
| WCS358 | Prototroph | 14 |
| WCS358.E9 | pfrA::Tn3Gus | 52 |
| VM119 | pfrI::Tn5 | 51 |
| Plasmids | ||
| pQE60 | Expression vector; ColE1 replicon, PT5lacO RBSII Apr | Diagen |
| pDMI,1 | Repressor plasmid; p15A replicon, lac1q Kmr | 6 |
| pRK2013 | Helper plasmid; ColE1 replicon, Kmr Mob+ Tra+ | 12 |
| pMP190 | Broad-host-range, low-copy-number promoter probe vector; IncQ replicon, lacZ Cmr Tra− | 47 |
| pMP220 | Broad-host-range, low-copy-number promoter probe vector; IncP replicon, lacZ Tcr Tra− | 47 |
| pPV51 | Promoter of pvdA pyoverdin synthesis gene cloned into pMP220 | 20 |
| pMP190::PpvdD | Promoter of pvdD pyoverdin synthesis gene cloned into pMP190 | 8 |
| pMP190::PpvdE | Promoter of pvdE pyoverdin synthesis gene cloned into pMP190 | 8 |
| pBRXB | 1.8-kb XhoI-BamHI fragment containing pvdS ligated to pBR322 | 8 |
| pPvdS6H | The 564-bp pvdS coding sequence ligated to the NcoI-BglII sites of pQE60 in frame to the His6 coding sequence | This study |
| pPvdSF | The 564-bp pvdS coding sequence ligated to the NcoI-HindIII sites of pQE60 in frame to the FLAG coding sequence | This study |
| pPvdSWT | The 564-bp pvdS coding sequence ligated to the NcoI-HindIII sites of pQE60 | This study |