TABLE 3.
Effects of pfrA and pfrI mutations on the activity of pvd::lacZ gene fusions in P. putida WCS358
| Strain | Genomic mutation | Plasmid | LacZ activitya
|
|
|---|---|---|---|---|
| −Fe(III) | +Fe(III) | |||
| WCS358 | pMP220 | 168 | 132 | |
| pMP190 | 52 | 38 | ||
| pPV51 | 11,621 | 141 | ||
| pMP190::PpvdD | 2,161 | 35 | ||
| pMP190::PpvdE | 4,230 | 41 | ||
| WCS358.E9 | pfrA::Tn3Gus | pMP220 | 131 | 110 |
| pMP190 | 98 | 75 | ||
| pPV51 | 92 | 101 | ||
| pMP190::PpvdD | 101 | 113 | ||
| pMP190::PpvdE | 116 | 109 | ||
| VM119 | pfrI::Tn5 | pMP220 | 129 | 113 |
| pMP190 | 95 | 78 | ||
| pPV51 | 93 | 101 | ||
| pMP190::PpvdD | 110 | 118 | ||
| pMP190::PpvdE | 120 | 115 | ||
a
Determined in culture lysates of P. putida grown overnight in DCAA [−Fe(III)] and DCAA supplemented with 100 μM FeCl3 [+Fe(III)]. Units of activity are as defined by Miller (28) and correspond to means of four determinations.