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Effect of the algR2 mutation on the activity of the pvdA::lacZ gene fusion in P. aeruginosa 8830
| Strain | Genomic mutation | Plasmid | LacZ activitya
|
|
|---|---|---|---|---|
| −Fe(III) | +Fe(III) | |||
| 8830 | his-1 | pMP220 | 48 | 50 |
| pPV51 | 2,151 | 38 | ||
| 8830R2::Cm | his-1 algR2::Cm | pMP220 | 38 | 35 |
| pPV51 | 2,200 | 34 | ||
Determined in culture lysates of P. aeruginosa grown in IFKB [−Fe(III)] and IFKB supplemented with 100 μM FeCl3 [+Fe(III)]. Units of activity are as defined by Miller (28) and correspond to means of four determinations.
