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. Author manuscript; available in PMC: 2022 Sep 5.
Published in final edited form as: Biochemistry. 2022 Apr 28;61(10):833–842. doi: 10.1021/acs.biochem.2c00096

Figure 1.

Figure 1.

CMCT followed by NaHCO3 treatment detects nine Ψ nucleotides in 23S rRNA of the 50S and the only Ψ in 16S rRNA of the 30S. (A,B) Background corrected rates of mutations were calculated as explained in the Materials and Methods section of the paper. Only the U residues are shown in the x-axes. The blue circles and the red diamonds are the data from two different biological samples. There are nine Ψ nucleotides in 23S rRNA of the 50S large subunit. We are able to detect all of them (A). The mutation rates for m3Ψ, as explained in the Results and Discussion section of the paper, are not shown in this figure (A). There is only one Ψ in 16S rRNA, which our chemical modification and mutation calculation methods also detect (B).