Figure 4.

OH⁵C is not present at a significant level in the 35S and 45S intermediates’ 23S rRNA, whereas m²A is incorporated into 23S rRNA before the 35S and 45S intermediates are populated in cells. The average mutation rates for OH⁵C and m²A were calculated, as explained in Materials and Methods section of the paper. The average mutation rates for the 35S and 45S intermediates and the native 50S large subunit are shown in green, blue, and red, respectively. The data are the averages of two biological replicates, and the errors are the standard deviations from the averages. The extent of the OH⁵C modification in the 35S and 45S intermediates is significantly smaller than the extent of the OH⁵C modification in the native 50S. On the other hand, the extent of m²A modification in the 35S and 45S intermediates is similar to the extent of m²A modification in the native 50S.