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. Author manuscript; available in PMC: 2022 Sep 5.
Published in final edited form as: Biochemistry. 2022 Apr 28;61(10):833–842. doi: 10.1021/acs.biochem.2c00096

Figure 5.

Figure 5.

KMnO4 treatment accurately detects the OH5C. Mutations rates after treating the sample with KMnO4 were calculated, as explained in the Materials and Methods section of the paper. Only C nucleotides are shown in the x-axes. The red diamonds and blue circles are the data from treating the rRNA with KMnO4 for 3 or 6 min, respectively. (A) Mutation rates of 23S rRNA from the native 50S large subunit treated with KMnO4. KMnO4 treatment detects the single OH5C modification in 23S rRNA of the native 50S large subunit. (B) Mutation rate of 16S rRNA from 30S treated with KMnO4. No OH5C is present in 16S rRNA of the 30S, and we do not observe false positive OH5C in this molecule.