TABLE 1.

Tetracycline resistance levels conferred by TetA(K) motif C mutants^a

Plasmid	Mutation	IC (μg/ml)
pBluescript II SK		5
pBR322		96
pSK4646		48
G151 mutations
pSK4591	G151R	5
pSK4592	G151V	10
pSK4594	G151E	5
pSK4595	G151D	5
pSK4596	G151L	5
pSK4597	G151K	5
pSK4598	G151A	48
pSK4599	G151S	48
G155 mutations
pSK4400	G155D	5
pSK4538	G155A	48
pSK4539	G155R	5
pSK4541	G155P	5
pSK4543	G155S	48
pSK4544	G155T	5
pSK4549	P155S-P156S	32
P156 mutations
pSK4551	P156A	5
pSK4552	P156R	5
pSK4555	P156L	5
pSK4556	P156T	5
pSK4559	P156V	5
pSK4560	P156N	5
pSK4561	P156Q	5
pSK4562	P156G	12
pSK4563	P156H	5
pSK4564	P156I	5
pSK4565	P156K	5
pSK4566	P156S	5
G159 mutations
pSK4568	G159A	8
pSK4570	G159R	5
pSK4571	G159D	5
pSK4572	G159N	5
pSK4573	G159E	5
pSK4574	G159P	5
pSK4575	G159S	8
pSK4576	G159T	5
pSK4577	G159V	8
G160 mutations
pSK4579	G160V	10
pSK4580	G160T	5
pSK4581	G160L	5
pSK4582	G160I	5
pSK4583	G160E	5
pSK4584	G160Q	5
pSK4585	G160R	5
pSK4586	G160A	48
pSK4588	G160S	32

^aAll mutants were constructed by site-directed mutagenesis procedures, with the exception of the hydroxylamine-generated G155 mutation of pSK4400 and the second-site suppressor mutation of pSK4541, pSK4549 (see Materials and Methods for details).