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. 2022 Aug 29;2022:6062236. doi: 10.1155/2022/6062236

Figure 4.

Figure 4

Upregulation of miR-29a-3p or miR-122-5p attenuates the NK cell-mediated cytotoxicity to ccRCC cells. (a–d) ACHN and Caki-1 cells were infected with NC mimic, miR-122-5p mimic, or miR-29a-3p mimic as indicated, and relative miRNA expression was assessed by qRT-PCR. (e–h) Calcein release assay to evaluate the NK cell cytotoxicity towards ACHN and Caki-1 cells as indicated. (i–l) Quantification of conjugated polarized perforin NK cells. (m–p) Conjugation assay to assess the formation of NK cells and infected ACHN or Caki-1 cell conjugates (n = 3; ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001).