Figure 5.

circFOXO3 regulates the NK cell-mediated cytotoxicity towards ccRCC cells via interacting with miR-29a-3p and miR-122-5p. (a–d) Cell models were constructed by infecting LV-NC, LV-circFOXO3, LV-circFOXO3+miR-122-5p mimic, or LV-circFOXO3+miR-29a-3p mimic into ACHN or Caki-1 cells as indicated, and infection efficiencies were determined by qRT-PCR. (e, f) The proliferation of transfected ACHN cells was measured by EdU. (g, h) Apoptosis of transfected ACHN cells was assessed by flow cytometry. (i–l) Calcein release assay to evaluate NK cell cytotoxicity towards ACHN and Caki-1 cells as indicated. (m–p) Quantification of conjugated polarized perforin NK cells. (q–t) Conjugation assay to assess the conjugate formation of NK cells and infected ACHN or Caki-1 cell conjugates (n = 3; ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001).