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. 2022 Aug 29;2022:6062236. doi: 10.1155/2022/6062236

Figure 6.

Figure 6

circFOXO3 expression is transcriptionally regulated by KLF16. The upstream transcriptional regulators of circFOXO3 were predicted using NCBI (https://www.ncbi.nlm.nih.gov/), UCSC (http://genome.ucsc.edu/), and JASPAR (http://jaspar.genereg.net/) datasets. Relative expression of circFOXO3 in (a) ACHN or (b) Caki-1 cells pretransfected with the indicated siRNAs quantified by qRT-PCR. (c) Binding sites of the FOXO3 promoter and KLF16 were predicted by the JASPAR dataset (relative profile score threshold 95%). Relative enrichment of FOXO3 promoter regions (P1-P4) in anti-IgG or anti-KLF16 bounds was analyzed by qRT-PCR in (d) ACHN or (e) Caki-1 cells using the IgG group as the control. (f, g) The interaction between KLF16 and FOXO3 promoter (P1/P2) regions was assessed by a luciferase reporter gene assay. (h) Expression of KLF16 in thirty pairs of ccRCC tissues measured by qRT-PCR (n = 3; ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001).