Figure 1.

Identification of ADSCs and the effect of KGN on the chondrogenic differentiation of ADSCs. (a) Flow cytometry was used to identify the surface antigens CD73, CD90, and CD45 of ADSCs. (b–c) Alcian blue staining was used to evaluate the chondrogenic differentiation ability of ADSCs in each group. (d–e) Toluidine blue staining was used to evaluate the chondrogenic differentiation of ADSCs in each group. (f) Western blot was used to detect the protein expression levels of Aggrecan, Collagen III, Collagen II, and SOX9 in ADSCs of each group, and grayscale analysis was performed. ^∗P < 0.05, ^∗∗P < 0.01 vs. 100 nM group.