TABLE 3.

Relative yield^a and specific activity^b of pep-tRNA hydrolase preparations of transformed strains

Strainc type	Untransformed (S30)		Transformed
			S30		DEAE		CMd sp act
	Concn	Sp act	Concn	Sp act	Concn	Sp act
pth(wt)	0.60 (±0.15)	6.83	18.00 (±3.2)	8.33	120 (±14.4)	8.75	6.40
pth(rap)	0.64 (±0.15)	0.46	19.20 (±4.1)	0.027	129 (±23.2)	0.03	0.04
pth(Ts)	0.12 (±0.02)	7.5	0.48 (±0.1)	6.04	1.70 (±0.2)	8.82

^aThe Pth concentration (nanograms of Pth per microgram of total protein) was estimated as described for Fig. 1. The numbers represent the means, and the values in parentheses are the estimated standard deviations in assays of four extracts. 

^bIn picomoles of substrate per minute per microgram of Pth protein. 

^cFor transformed strains, the pth alleles in the chromosome and in the constructs were identical: C600 pth(wt)/pGI01; C600 pth(rap)/pGI02 and C600 pth(Ts)/pGI03. 

^dCM, carboxymethyl cellulose.