Fig. 3.

Human myometrial organoids derived from CD44/Stro1 +  + stem cells exhibit a significant response to ovarian steroid hormones in vitro. On day 7, the organoids derived from human MMSC and UFSC were treated with estradiol (E2, 10 ng/ml) or progesterone (P4, 10 ng/ml) for 72 h. A Representative phase-contrast microscopy pictures are exhibiting the effects of E2 and P4 on the growth of MMSC-derived MyoN and UF organoids. B The bar graph is a quantitative representation of the difference in surface area of the MMSC-derived MyoN and UF organoids in response E2 and P4. Data are from five independent (n = 5) experiments performed for each individual group. The bar graphs represent the mean ± SEM of results (n = 5). Asterisks (*) represent unpaired Student’s t test, *p ≤ 0.01, **p ≤ 0.001, ***p ≤ 0.0001, NS, not significant