Obesity reversal decreases global DNA methylation levels in normal mammary tissue. Pairwise comparisons of DNA methylation levels between (A) the obese and nonrestricted low-fat diet (LFD) groups, (B) the obese and low-fat calorie restricted (LFCR) groups, and (C) the obese and Mediterranean-style calorie restricted (MCR) groups are shown, expressed as the mean change in percent methylation and with a differentially methylated region (DMR) defined as a genomic site with an average absolute methylation value change of ≥15% and an FDR adjusted P<0.05 (n=3–5 mice/group). Locations of the DMR for each comparison, expressed as the number of DMR and percentage of DMR in each location, are also shown.