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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Cancer Prev Res (Phila). 2022 Sep 1;15(9):581–594. doi: 10.1158/1940-6207.CAPR-22-0113

Figure 7.

Figure 7.

Obesity reversal by calorie restriction promotes changes in the expression of mouse mammary tissue genes that are enriched in the breast tissue of calorie-restricted women. (A) The top 100 significant differentially expressed genes (DEG) from 4 pairwise comparisons (low-fat calorie-restricted (LFCR), Mediterranean-style calorie-restricted (MCR), intermittent calorie restricted (ICR), and nonrestricted low-fat diet (LFD) mice versus obese, n=3–5 mice/group) were assessed by Gene Set Enrichment Analysis (GSEA) for enrichment in a gene set from the breast tissue of women who were overweight/obese and randomized to a chronic energy restriction (CER) regimen versus usual diet (control) (GSE66159). GSEA was also used to assess (B) all DEG from the comparison of all-CR mice versus obese mice, excluding any DEG from the comparison of nonrestricted LFD versus obese mice, and (C) all DEG from the comparison of nonrestricted LFD versus obese mice, excluding any DEG from the all-CR versus obese comparison, for enrichment in the same human dataset.