Table 1.

Nutrient information on all diets

							ICR
		Control	DIO	LFD	LFCR	MCR	5-day	2-day	Average
Kcal density of diet (kcal/g)		3.85	5.21	3.85	3.78	4.13	4.31	3.36	NA
Macronutrient distribution (% of total kcal)	Carbohydrate	70	20	70	57	41	44	27	39
	Protein	20	20	20	29	29	25	50	32
	Fat	10	60	10	14	30	31	23	29
Average total daily kcal †		8.78	10.5	8.26	6.10	6.10	7.50	2.61	6.10
Macronutrient kcal ‡	Carbohydrate	6.15	2.10	5.78	3.48	2.50	3.30	0.70	2.38
	Protein	1.76	2.10	1.65	1.77	1.77	1.88	1.30	1.95
	Fat	0.88	6.30	0.83	0.85	1.83	2.33	0.60	1.77
Fatty acid distribution (% of total kcal)	SFA	2.0	19.2	2.0	3.29	6.93	7.10	5.31	6.59
	MUFA	3.0	21.0	3.0	4.16	15.0	15.6	11.5	14.4
	PUFA	5.0	19.8	5.0	6.55	8.04	8.34	6.21	7.73
	n-3 FA	0.46	1.0	0.46	0.69	0.60	0.62	0.46	0.57
Fatty acid kcal ¥	SFA	0.18	2.02	0.17	0.20	0.42	0.53	0.14	0.42
	MUFA	0.26	2.21	0.25	0.25	0.92	1.17	0.24	0.90
	PUFA	0.44	2.08	0.41	0.40	0.49	0.63	0.16	0.50
	n-3 FA	0.04	0.11	0.04	0.04	0.04	0.05	0.01	0.04

^†Average total kcal/mouse/day consumed

^‡average kcal/mouse/day consumed from each macronutrient

^¥average kcal/mouse/day consumed from different categories of fatty acid, all calculated from week 15 through the end of study. The Control and DIO mice were maintained on the above diets throughout the study. LFD, LFCR, MCR, and ICR mice were switched to the above diets after 15 weeks on the DIO diet, then remained on those diets through the end of study. LFCR and MCR mice received daily pellets equivalent to 70% of the average kcal/day consumed by the control mice in the previous week. ICR mice on average received the same amount of kcal/day as the LFCR and MCR mice. Abbreviations: DIO, diet-induced obesity; ICR, intermittent calorie restricted; kcal, kilocalorie; LFCR, low-fat calorie restricted; MCR, Mediterranean calorie restricted; MUFA, monounsaturated fatty acids; n-3 FA, omega-3 fatty acids; PUFA, polyunsaturated fatty acids; SFA, saturated fatty acids.