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. Author manuscript; available in PMC: 2023 Mar 2.
Published in final edited form as: Cancer Res. 2022 Sep 2;82(17):3158–3171. doi: 10.1158/0008-5472.CAN-22-0170

Figure 4. Palovarotene alters the BMP4-induced transcriptome.

Figure 4.

(A) Venn diagram of number of genes whose expression levels are upregulated by BMP4 and downregulated by Palo in 2H11 cells. Pathway analyses of genes upregulated by BMP4 (B), downregulated by Palo after 48 h (C), and up-regulated by BMP4 but down-regulated by Palo (Palo-regulated genes) (D). (E) Transcription factors upregulated by BMP4 and downregulated by Palo. qRT-PCR for indicated mRNAs. (F) Category of BMP4-inducible secreted proteins that are inhibited by Palo. (G) BMP4-stimulated secreted proteins (termed “osteocrines”) (12) that are decreased by Palo. (H) qRT-PCR for select mRNAs in (G) treated as indicated.