. 2000 Mar;182(6):1541–1548. doi: 10.1128/jb.182.6.1541-1548.2000

TABLE 1.

Bacteria and plasmids used in this study

Bacterial strain or plasmid	Relevant genotype, phenotype, or characteristic^a	Source or reference
A. tumefaciens
NT1	Ti plasmid-cured derivative of C58, contains pAtC58	54
C58C1RS	Ti plasmid-cured derivative of C58; Rif^r Str^r; contains pAtC58	49
C58C1EC	Ti plasmid-cured derivative of C58; Ery^r Chl^r; contains pAtC58	32
UIA5	Ti plasmid-cured derivative of GMI9017; Rif^r Str^r; lacks pAtC58	11, 42
NT1(pZLR4)	AAI bioindicator strain; Cb^r	9
E. coli
DH5α	supE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1	43
HB101	hsdS20 recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 supE44 Str^r	43
HB101Nx	hsdS20 recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 supE44 Str^r Nal^r	2
HB101Rif^R	hsdS20 recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 supE44 Str^r Rif^r	11
S17-1	pro (r⁻ m⁺) Mob⁺ Amp^r Chl^r Tmp^r Str^r	19
Plasmids
pTiC58ΔaccR	accR deletion mutant of pTiC58; Tra^cacc^c	11
pDCKI41	traG::Tn3HoHo1 insertion mutant of pTiC58ΔaccR; Tra⁻	19
pJB3	IncP1 cloning vector; Mob⁺ Ap^r Cb^r	5
pRK415	IncP1 cloning vector; Mob⁺ Tc^r	27
pDSK519	IncQ cloning vector; Mob⁺ Km^r	27
pMMB67HE	IncQ cloning vector; Mob⁺ Ap^r Cb^r	22
pVK225	Cosmid clone of virCDE region from pTiA6NC	26
pDB126	Tra1 core region and Tra2 region of RP4 cloned in ColD vector; Tra⁺ Cm^r	2
pDB127	traG deletion mutant of pDB126; Tra⁻ Cm^r	2
pBS141	traG of RP4 cloned in pMMB67HE; IncQ Ap^r Cb^r	2
pBS141K187T	K187→T mutant of traG in pBS141; IncQ Ap^r Cb^r	2
pBS141K209T	K209→T mutant of traG in pBS141; IncQ Ap^r Cb^r
pBS141E211Q	E211→Q mutant of traG in pBS141; IncQ Ap^r Cb^r	2
pBS141D449N	D449→N mutant of traG in pBS141; IncQ Ap^r Cb^r	2
pBS141K456T	K456→T mutant of traG in pBS141; IncQ Ap^r Cb^r	2
pDCE20	traCDG operon of pTiC58 cloned in pRK415; IncP1 Tc^r	19
pHL142	virD4 of pTiA6NC cloned in pDSK519; IncQ Km^r	This study
pPLtrb	traI/trb region of pTiC58 cloned in pJB3; IncP1 Ap^r Cb^r	This study
pPLtrb-DB	Tra1 core region of RP4 cloned into pPLtrb; IncP1 Ap^r Cb^r	This study
pZLQR	traR of pTiC58 cloned in pBBR1MCS2; Inc? Km^r	34
pZLQRF	traF of pTiC58 cloned into pZLQR; Inc? Km^r	This study

Antibiotics: Amp and Ap, ampicillin; Cb, carbenicillin; Chl and Cm, chloramphenicol; Ery, erythromycin; Km, kanamycin; Nal, nalidixic acid; Rif, rifampin; Str, streptomycin; Tc, tetracycline; Tmp, trimethoprim. Other symbols: acc^c, constitutive expression of acc; Inc?, unknown incompatibility group; Mob, mobilizable; Tra⁺, conjugation proficient; Tra⁻, conjugation deficient; Tra^c, constitutive for conjugation.