TABLE 2.

TraG of RP4 allows pTiC58 to mobilize IncQ plasmids^a

IncQ plasmidb	traG allelec	Mobilization frequencyd	Ti plasmid coinheritancee
pDSK519	None	<10−9	NA
pMMB67HEf	None	<10−9	NA
pBS141	Wild type	3 × 10−7	0.04
pBS141K187T	K187T	<10−9	NA
pBS141K209T	K209T	1.6 × 10−8	0.14
pBS141E211Q	E211Q	<10−9	NA
pBS141D449N	D449N	<10−9	NA
pBS141K456T	K456T	5.5 × 10−7	0.28

^aA. tumefaciens UIA5(pTiC58ΔaccR) was used as the donor in filter matings with strain C58C1EC as described in Materials and Methods. 

^bAll plasmids are derivatives of RSF1010 and contain the IncQ oriT and mobilization genes mobA, mobB, and mobC. 

^cSee Fig. 2. 

^dExpressed as transconjugants obtained per input donor. The full set of matings was repeated twice with indistinguishable patterns of results. The data from a single set of matings conducted in parallel on the same day are presented. 

^eAssessed by picking 100 transconjugant colonies to AT minimal medium containing nopaline as the sole carbon source as described in Materials and Methods. NA, not applicable. 

^fVector used for construction of the pBS141 series of plasmids.