TABLE 3.

TraG_RP4-mediated mobilization from the IncQ relaxosome does not interfere with Ti plasmid transfer but may restrict transfer to the same recipient

IncQ plasmid	Transfer frequencya of:
	pTiC58ΔaccRb	Coinheritance of IncQ plasmidc	IncQ derivatived	Coinheritance of Ti plasmide
None	7.1 × 10−3	NA	NA	NA
pDSK519	6.7 × 10−2	<0.01	<10−8	NA
pBS141	1.2 × 10−2	<0.01	3 × 10−6	0.12

^aExpressed as transconjugants per input donor. The experiment was repeated once with similar patterns of results. NA, not applicable. 

^bTransconjugants were selected on AT minimal medium supplemented with chloramphenicol and erythromycin and containing nopaline as the sole carbon source. 

^cAssessed by picking 100 transconjugant colonies to nutrient agar containing kanamycin (pDSK519) or carbenicillin (pBS141) in addition to chloramphenicol and erythromycin. 

^dTransconjugants were selected on nutrient agar containing kanamycin (pDSK519) or carbenicillin (pBS141) in addition to chloramphenicol and erythromycin. 

^eAssessed by picking 100 colonies to AT minimal agar containing nopaline as the sole carbon source in addition to chloramphenicol and erythromycin.