TABLE 4.
VirD4 couples transfer of an IncQ plasmid but not the Ti plasmid to the Ti plasmid mating bridge
| Source of coupling proteina | Coupling protein | Frequency of transferb of:
|
||
|---|---|---|---|---|
| pDCKI41 to A. tumefaciens | IncQ plasmid to:
|
|||
| A. tumefaciens | E. coli | |||
| None | None | <10−8 | NA | NA |
| pDCE20 | TraGpTiC58 | 2.3 × 10−3 | NA | NA |
| pDSK519 | None | <10−8 | <10−8 | <10−8 |
| pHL142 | VirD4 | <10−8 | 1.9 × 10−5 | 1.4 × 10−6 |
a
All A. tumefaciens donors harbored pDCKI41 (traG::Tn3HoHo1) in addition to the recombinant plasmid providing the coupling protein. NA, not applicable.
b
Expressed as transconjugants obtained per input donor. The experiment was repeated three times with indistinguishable patterns of results.