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. 2022 Sep 5;13:5211. doi: 10.1038/s41467-022-32868-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Schematic of the assessment of Msx1⁺ SSCs subset in differentiation hierarchy by Prx1-lineage tracing mice. b, c HE staining of newly formed calvarium tissues in skull bone injury at 2 weeks and 4 weeks after surgery, respectively. d–g Coronal sections of the calvarium tissues from Prx1^Cre; Rosa^tdTomato mice co-immunostained with Msx1 and Runx2 (d); Msx1 and OCN (e); Msx1 and ACAN (f); Msx1 and COL2 (g); Scale bars: low magnification: 30 μm, high magnification: 10 μm. At least three times of experiments were repeated independently.