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. 2022 Sep 5;13:5228. doi: 10.1038/s41467-022-32124-3

Fig. 5. Overexpressing the ankyrin repeat domain (ANK) in WT plants reduces arbuscule abundance.

Fig. 5

WT roots were transformed with 35Spro:VPY (n = 7 root systems), 35Spro:VAP (n = 4 root systems), 35Spro:ANK (n = 7 root systems), or a negative control construct, 35Spro:GFP (n = 4 root systems) and colonized with D. epigeae. a Percentage of infection units containing arbuscules in transgenic roots. Different letters indicate statistically significant differences using one-way ANOVA, p < 0.05. Lines in boxplots represent the median value, box limits represent the upper and lower quartiles, whiskers represent 1.5 times the interquartile range. Confocal image of an infection unit in a WGA-AlexaFluor488 stained root expressing 35Spro:GFP (b) or 35Spro:ANK (c). Images show typical infection units in the GFP or ANK overexpressing roots. Scale bars, 250 μm. Images are overlays of fluorescence and DIC.