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. 2022 Sep 5;5:912. doi: 10.1038/s42003-022-03889-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Representative images hybridised with a 35S-labelled oligodeoxynucleotide probe complementary to mRFP1 in the SON, dpPVN, and mPVN of female OXT-mRFP1 transgenic rats in the OVX + oil-only (n = 6), OVX + low E2 (n = 6), and OVX + high E2 (n = 6) groups, respectively. The scale bar indicates 100 μm. b mRFP1 mRNA in the SON, dpPVN, and mPVN of the rats (OVX + oil-only, OVX + low E2, and OVX + high E2 groups). The data are presented as the mean ± SEM (one-way ANOVA) (^**P < 0.01, ^*P < 0.05, compared with OVX + oil only group). c Regression analysis for plasma E2 and changes in mRFP1 mRNA levels in the SON, dpPVN, and mPVN. The regression line and probability value for the slope are shown. The statistical significance of the slope was set at P < 0.05. d Regression analysis for plasma OXT and changes in mRFP1 mRNA levels in the SON, dpPVN, and mPVN. The regression line and probability value for the slope are shown. The statistical significance of the slope was set at P < 0.05.