Fig. 6. OT alleviated hyperalgesia in a neuropathic pain model via neuronal pathway.

a The Seltzer surgery was performed via dorsal approach. Experimental procedures administering CNO after the Seltzer surgery (b) and OT receptor (OTR) antagonist treatment (c). Results of the manual von Frey (d) and hot plate tests (e) after the s.c. injection of Saline or CNO (1 mg kg⁻¹) in the Seltzer model (n = 9 rats, each). Data are represented as mean ± SEM. **P < 0.01 vs. Saline at the same time point. ^#P < 0.05; ^##P < 0.01 vs. CNO at 0 min. Results of the manual von Frey (f) and hot plate tests (g) after the s.c. injection of CNO (1 mg kg⁻¹) pretreatment with L-371,257 dissolved in DMSO [10 mg kg⁻¹] for OTR antagonist i.p., Atosiban dissolved in saline [1 μg μL⁻¹] for OTR antagonist i.t., and/or vehicle in the Seltzer model (n = 9 rats, each). Data are represented as mean ± SEM. **P < 0.01 vs. Vehicle i.p. + Vehicle i.t., ^#P < 0.05; ^##P < 0.01 vs. OTR antagonist i.p. + Vehicle i.t. at the same time point. See also Supplementary Fig. 7.