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Sucrose gradient sedimentation analysis of the XpsN protein in XC1701 on a 25 to 61% (wt/wt) sucrose gradient. The top two panels are immunoblots of fractions 7 to 34 collected from the gradient (top left) detected with antibody against the XpsN protein and with antibody against the E. coli OmpA protein (OprF). In the bottom panel, open bars represent succinate dehydrogenase (SDH) activity and solid squares represent density.
