Coimmune precipitation of XpsN with XpsD in the parental strain XC1701. (A) Triton X-100 membrane extracts precipitated with antibody against the XpsN protein and detected on a Western blot with anti-XpsDN antibody (1:1,000 dilution). (B) Triton X-100 membrane extracts precipitated with anti-XpsDN antibody and detected on a Western blot with antibody against the XpsN protein (1:250 dilution). Samples were loaded in the following order: lane 1, XC1701 (xpsN+ xpsD+); lane 2, XC1708 (xpsN+ xpsD); lane 3, XC1709 (xpsN xpsD+); lane 4, no membrane extract.