FIGURE 10.

Western blot analysis of the CCS protein in lysates of HepG2 cells in the presence and absence of the flavonoids 3-HF or QT and Cu(II). HepG2 cells were stimulated with 20 μM flavonoid with and without 50 μM CuSO₄ and incubated for 24 h. Cell lysates were collected, and Western blot analysis was performed using antibodies specific for CCS with α-tubulin as a control. Western blot images for the remaining assessed flavonoids are shown in Supplementary Figure S5.