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. 2022 Aug 23;10:972198. doi: 10.3389/fchem.2022.972198

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Copyright © 2022 Lee and Heffern.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CCA fluorescence intensity measurements for determining the effects of flavonoids on the time-dependent generation of ^•OH. Flavonoid effects were compared to (A) Cu(II) addition alone, with (B) QT shown as an example. 2.5 mM CCA and 50 μM ascorbic acid were used for all experiments. The calculated area under the curve over the course of 90 min was measured for flavonoid solutions. (C) EC and (D) QT effects are shown as representative plots in the presence (blue) and absence (red) of Cu(II).