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. 2022 Sep 5;5(11):e202201570. doi: 10.26508/lsa.202201570

Figure 3. The effect of increasing phospho-mimetic mutations on Ci activity in vivo.

Figure 3.

(A, B, C, D, E, F, G, H, I, J) Control (A, A”) or wing discs expressing UAS-Myc-Ci-PKA_WT (B, B”), UAS-Myc-Ci-PKA_SD1 (C, C”), UAS-Myc-Ci-PKA_SD12 (D, D”), or UAS-Myc-Ci-PKA_SD123 (E, E”) under the control of C765 Gal4 driven from larvae grown at 25°C were immunostained to the expression of Ci (white in A, B, C, E; red in A”, B”, C”, D”, E”) and Ptc (white in A’, B’, C’, D’, E’; green in A”, B”, C”, D”, E”). Insets in D” and E” are large magnification views of the regions outlined in D’ and E’, respectively, which demarcate A-compartment cells distant from the A/P boundary. (F, G, H, I, J) Adult wings of the indicated genotypes are shown. Arrows in (G) and (H) indicate the gaps on wing vein 4. Scale bars are 50 μM for wing discs and 500 μM for adult wings.